Case · SR-LSA-001 · published
A 6-year-old Golden Retriever presented with bilateral submandibular lymph-node enlargement and was diagnosed by FNA + flow cytometry as multicentric B-cell lymphoma (CD21+, intermediate-large). We ran a pre-treatment lymph-node biopsy through DarkScan V2 + Sci-JEPA to establish a baseline immune-relevant ranking before CHOP induction.
Patient (de-identified)
The findings, in plain language
The top neoantigen, FNLPETVTL, arose from a BCL6 mutation in the lymphoma cells (DLA-88 IC50 31 nM). Three of the top five rankings came from BCL6 and MYC variants — consistent with the canine DLBCL-like biology described in the comparative oncology literature.
Sci-JEPA cross-referenced the canine ranking against the consensus human DLBCL neoantigen catalog and flagged structural homology between our top epitope and a published human DLBCL neoantigen variant. This is one of the use cases the atlas is designed for: helping a comparative oncologist quickly see where the canine signal aligns with the human field.
Why a pre-CHOP baseline matters: when this dog inevitably enters remission and then (statistically) relapses, we’ll be able to sequence the relapsed sample, re-rank, and compare. That delta is scientifically the most interesting moment — and impossible to study without the pre-treatment baseline we ran here.
Top ranked epitopes (SR-LSA-001 pre-CHOP)
| Rank | Sequence | Source | DLA IC50 (nM) | RNA TPM | Human analog |
|---|---|---|---|---|---|
| 1 | FNLPETVTL | BCL6 mutation | 31 | 84 | DLBCL (homolog) |
| 2 | KLPGDEFKA | MYC rearrangement | 47 | 118 | Burkitt-like |
| 3 | RTFRSPVVT | BCL6 mutation | 55 | 76 | DLBCL (homolog) |
| 4 | QEMRYNPAL | MYC rearrangement | 62 | 104 | Burkitt-like |
| 5 | HTSFEYGNE | EZH2 mutation | 70 | 39 | DLBCL (homolog) |
Full 10-epitope ranking, alignment provenance, and source-variant VCFs available in the clinical report on request.
Provenance
Includes full neoantigen ranking, alignment provenance, the human-canine atlas cross-references, immunophenotype panel, and a longitudinal plan for post-CHOP re-sequencing. Free for credentialed veterinarians and academic researchers.
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