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DarkScan V2 · production

From a canine biopsy to a ranked vaccine in hours.

DarkScan V2 is our neoantigen discovery pipeline — an intelligent funnel that reduces ~700–1,000× the candidate space vs naive enumeration without sacrificing recall. Every canine vaccine SciRouter ships starts here.

The funnel

Why a smart pipeline beats brute force.

Stage 1

~10⁵–10⁶

Variants called

raw tumor vs normal variants

Stage 2

~10⁴

Expressed in tumor

RNA-seq filter

Stage 3

~10³

MHC-presentable

DLA-88 / DLA-12 binding (canine)

Stage 4

~10²

Non-self

host proteome subtraction

Stage 5

10–30

Top ranked

Sci-JEPA + immunogenicity scoring

Each stage applies a learned or rule-based filter — the speedup comes from doing inexpensive filters first and reserving Sci-JEPA + immunogenicity inference for the ~10³ candidates that survive the upstream funnel.

The numbers

What the speedup buys.

Speedup~700–1,000×

vs. naive O(N×alleles) enumeration on the same hardware. The funnel structure does the work.

Turnaround~6 hours

from raw VCF + RNA expression to a ranked peptide list with rationale. A clinical case fits in a coffee + walk-the-dog cycle.

Cost per case~$8

compute amortized on RunPod A100 80GB. Orchestration runs on a $7/month Railway dyno. The asset-light story is real.

What lands on the vet's desk

Ranked candidates with rationale.

#
Peptide
Source
IC50 (nM)
Rationale
1
VSPSFSSTL
MAGEC1
26
CT-antigen, presented by HLA-B*27:05 (human corollary). Sci-JEPA flags pediatric-OS analog with high confidence.
2
YLLDDLLAR
RUNX2
142
Osteoblast TF; canine-expressed. Moderate-affinity DLA-88 presenter.
3
SLLPAIVEL
TP53 (R175H)
88
Hotspot mutation analog; cross-species literature support.

Excerpt from the SR-OST-001 case study (canine appendicular osteosarcoma, distal radius, 9-year-old Rottweiler).

DarkScan V2 is the engine. SR-OST-001 is the proof point.